Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Comparative performance of four LIT modes, Luminex, and ELISA

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Incubation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: Schematic diagrams illustrating the methodologies used in Luminex® Assay ( a ) and ELISA ( b ). Figure was created with MedPeer (medpeer.cn). c Dose dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and Luminex (red). d Dose dependent median fluorescence intensity and absorbance of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at different concentrations were compared between Standard LIT (black) and ELISA (red). Data presented as mean ± SD from three replicates. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, Fluorescence, Standard Deviation

Journal: Nature Communications

Article Title: Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array

doi: 10.1038/s41467-025-59390-1

Figure Lengend Snippet: a Dose-dependent median fluorescence intensity of IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF at various concentrations compared between Standard LIT assays with (red) and without (black) added serum. b Comparison of LODs for IL-1β, IL-4, IL-5, IL-6, IL-8, and GM-CSF with (pink) and without (blue) serum. c Spike recoveries for cytokines at high, medium, and low analyte concentrations. Linear regression plots comparing serum concentrations of IL-6 ( d ) and IL-8 ( e ) as determined by Luminex and LIT. Linear regression plots for IL-6 ( f ) and IL-8 ( g ) serum concentrations, determined by ELISA and LIT. Linear regression plots for IL-6 ( h ) and IL-8 ( i ) serum concentrations, determined by chemiluminescence and LIT. Data are presented as mean ± SD; n = 3 repeated tests. General data were analyzed with Origin 2021. The five-parameter logistic regression model was used to fit the standard curves. LOD and LOQ were defined as the concentrations corresponding to signal values of mean(blank) + 3σ(blank) and mean(blank) + 10σ(blank), respectively, where mean(blank) is the average signal and σ(blank) is the standard deviation of the blank measurements.

Article Snippet: Human IL-1β ELISA kit (NOVUS, VAL101), Human IL-4 ELISA kit (NOVUS, VAL123), Human IL-5 ELISA kit (NOVUS, VAL125), Human IL-6 ELISA kit (NOVUS, VAL102), Human IL-8 ELISA kit (NOVUS, VAL103), and Human GM-CSF ELISA kit (NOVUS, VAL124) were purchased.

Techniques: Fluorescence, Comparison, Luminex, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Journal of Biological Chemistry

Article Title: 5-Oxo-6,8,11,14-eicosatetraenoic Acid Stimulates the Release of the Eosinophil Survival Factor Granulocyte/Macrophage Colony-stimulating Factor from Monocytes

doi: 10.1074/jbc.m401537200

Figure Lengend Snippet: FIG. 5. Mediation of 5-oxo-ETE-induced eosinophil survival by monocyte-derived GM-CSF. Left, in control experiments, monocyte- depleted eosinophils were incubated for 48 h with vehicle (control (Con); open bar), 1 nM IL-5 (hatched bars), or 1 nM GM-CSF (GM; cross- hatched bars) in the presence or absence of monoclonal antibody against IL-5 (aIL5) or GM-CSF (aGM). Right, monocytes from four different donors were incubated for 24 h with either vehicle (open bar) or 1 M 5-oxo-ETE (5o; closed bars). The conditioned media (MCM) were then incubated for 60 min at 37 °C in the presence or absence of monoclonal antibody (20 g/ml) against IL-5 or GM-CSF. Aliquots (10 l) of the conditioned media were then incubated for 48 h with monocyte-depleted eosinophils from two different donors, and cell survival was determined by flow cytometry. The average percent survival for the two eosinophil preparations was calculated for each of the monocyte preparations, and n was taken to be equal to the number of monocyte donors (i.e. n 4). ***, p 0.001.

Article Snippet: The neutralizing monoclonal antibodies against recombinant human IL-5 and recombinant human GM-CSF were purchased from R&D Systems (Minneapolis, MN).

Techniques: Derivative Assay, Control, Incubation, Flow Cytometry

Journal: Journal of Biological Chemistry

Article Title: 5-Oxo-6,8,11,14-eicosatetraenoic Acid Stimulates the Release of the Eosinophil Survival Factor Granulocyte/Macrophage Colony-stimulating Factor from Monocytes

doi: 10.1074/jbc.m401537200

Figure Lengend Snippet: FIG. 6. 5-Oxo-ETE induces GM-CSF release from monocytes. A, monocytes were incubated for different times at 37 °C with either vehicle (control; E) or 1 M 5-oxo-ETE (G), and the amounts of GM-CSF in the media were determined by enzyme-linked immunosorbent assay as described under “Experimental Procedures.” B, monocytes were in- cubated for 24 h at 37 °C with either vehicle or different concentrations (1 pM to 100 nM) of either 5-oxo-ETE (G) or LTB4 (E), and the amounts of GM-CSF in the media were measured. The inset shows the responses to higher concentrations (10 nM to 10 M) of 5-oxo-ETE (G) and LTB4 (E). Because of limitations in the numbers of monocytes, only a limited number of concentrations of LTB4 were tested. C, purified eosinophils from three different donors were incubated for 48 h at 37 °C with either vehicle or different concentrations (10 fM to 100 pM) of recombinant human GM-CSF, and survival was assessed following staining with FITC-labeled annexin V and propidium iodide. *, p 0.05; **, p 0.01; ***, p 0.001.

Article Snippet: The neutralizing monoclonal antibodies against recombinant human IL-5 and recombinant human GM-CSF were purchased from R&D Systems (Minneapolis, MN).

Techniques: Incubation, Control, Enzyme-linked Immunosorbent Assay, Purification, Recombinant, Staining, Labeling